WebDetermine the time course of cycloheximide chase beforehand. There can be great variation among proteins. For an unfamiliar protein, it is recommended to start with a 4hour interval - and chase till 24 h. Cycloheximide may cause cytotoxicity to certain cells if cells are exposed to it over 2024 h. WebApr 18, 2016 · Cycloheximide Chase Analysis Protein Degradation Saccharomyces. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, …
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WebApr 10, 2024 · A cycloheximide (CHX) pulse-chase experiment was then performed to confirm that OTUD6B stabilizes the β-TrCP protein. To do so, β -TrCP with wild-type OTUD6B or OTUD6B C188S were co-transfected into HEK293T cells, and the stability of β -TrCP was found to be prolonged in the presence of wild-type OTUD6B but not OTUD6B … WebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... For an unfamiliar protein, it is recommended to start with a 4-hour interval and chase till 24 h. Cycloheximide may cause cytotoxicity to certain cells if cells … ctrl clothing uk
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WebJul 23, 2024 · In this study, we performed a cycloheximide (CHX) chase assay to measure the half-lives of approximately 30 human lysosomal membrane proteins (LMPs) and identified RNF152 and LAPTM4A as short-lived membrane proteins. The degradation of both proteins is ubiquitin dependent. RNF152 is a transmembrane E3 ligase that … WebApr 10, 2024 · Harsha Ganesan. “Sushmitha is an amazing researcher in various fields of cancer research including miRNA-protein interaction in Colon cancer. Even as an early carrier researcher, she produced ... Web2 days ago · Net interest income is projected to surge 36.5% to $19.06 billion, resulting in a net interest margin of 2.40%. Earnings per share (EPS) likely rose 30% year-over-year to $3.43, driven by a 36.5% ... earth \u0026 turf attachments