Csfv shimen strain

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August undefined, 2024

WebMar 13, 2024 · As shown in Fig. 4A,B, our method could detect the CSFV Shimen strain or the HCLV strain, while negative results were obtained with other virus strains. Three independent reproducibility tests ... WebThe CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I / SM) [18] and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number ...

Visual detection and differentiation of Classic Swine …

WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and … WebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF … hik connect india login

Construction of full-length infectious cDNA clones of …

WebClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. It has been proposed that lipid synthesis is essential for viral replication, and lipids are involved in viral protein maturation and envelope production. However, the specific crosstalk between CSFV and host cell lipid metabolism … WebApr 2, 2024 · A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and … hik connect illegal login

Classical swine fever virus triggers RIG-I and MDA5-dependent …

Characterization of the rescued CSFVs. (A) Growth kinetics of the...

WebDuplex PCR for distinguishing shimen and C-strain of CSFV A duplex PCR for distinguishing the Shimen strain from the C-strain was performed with a total volume of … WebJul 1, 2015 · The Shimen strain infection resulted in a significant activation of IFN regulatory factor IRF7 and suppression of IRF3. ... two classical swine fever virus (CSFV) strains, namely the highly virulent CSFV Shimen strain and the avirulent C strain (a vaccine strain), were employed, and the expression of 19 immune effector genes was … hik connect how toWebFeb 4, 2024 · Methods Viruses and cells. CSFV strains Shimen (GenBank: FJ598612.1) and HZ08 (GenBank: EF683627) were received from CATG lab,... Construction of … small usb keyboards with keypad

"WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) promoter and T7 promoter to enable rescue by either of the methods described above. To ensure integral and precise transcription of the genome, the genomic cDNA was flanked … " - Csfv shimen strain

Csfv shimen strain

Classical swine fever virus triggers RIG-I and MDA5 …

WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and RT-PCR. Six pairs of primers based on the published known sequence of CSFV Zj0801 (GenBank accession no. FJ529205) were designed for PCR analysis (Table ... WebNov 27, 2015 · The highly virulent CSFV Shimen strain (wt-Shimen) was used as a wild-type control in the experiment. EGFP-CSFV is an EGFP-tagged CSFV derived from pEGFP-CSFV [ 10 ]. vSM-HCLV3′UTR is a chimeric CSFV based on the wt-Shimen backbone with the 3′-UTR replacement by that of HCLV [ 8 ], which was generated in a way similar to …

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WebNov 19, 2024 · Then, the cells were stimulated with ultraviolet-light-inactivated CSFV (Shimen strain; MOI = 1) l. After 24 h of incubation at 37 °C, the cell-free supernatant was removed and detected using a commercially available porcine IL-4 and IFN-γ ELISA kit according to the manufacturer’s protocol. OD values were measured at 450 nm using a ... WebMar 14, 2024 · The Shimen strain is the standard virulent strain of CSFV in China , and induces severe immunopathological symptoms associated with hemorrhagic fever, thrombocytopenia, and lymphoid organ atrophy and …

WebAug 17, 2024 · In this study, we demonstrated that P108 and T109 on the E2 glycoprotein together with the E rns glycoprotein of the rabbit-adaptive C-strain confer adaptation to rabbits on the highly virulent CSFV Shimen strain by affecting viral entry during infection but do not attenuate the Shimen strain in pigs. Our results provide vital information on ... WebApr 23, 2014 · The CSFV Shimen and C strains were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (Beijing, China). SUVECs were cultured in 25-cm 2 tissue culture flasks at a density of 2 × 10 7 cells per flask. The Shimen and C strains of CSFV were added to cultures at an MOI of 10 TCID50 per cell when SUVECs were …

WebMar 8, 2024 · The Shimen strain of classical swine fever virus (CSFV) causes an especially infectious disease in domestic pigs known as classical swine fever (CSF), which has been listed as a highly contagious disease by the World Organisation for Animal Health [].Acute CSF is typically accompanied by haemorrhagic lymphadenitis and diffuse haemorrhage … WebSep 13, 2013 · In present study, we investigated the roles of RIG-I and MDA-5 in eliciting antiviral and inflammatory responses to CSFV shimen strain in Porcine alveolar …

WebMay 1, 2024 · The chimeric CSFV/BVDV fragment consisted of the E2 gene of BVDV and p7 gene and incomplete NS2–3 coding region of CSFV, which was obtained from overlap PCR amplification using the cDNAs of BVDV Hubei strain and CSFV Shimen strain as templates. The pCSFV/bE2 mutants were constructed using genetic approaches based …

WebJul 22, 2024 · The CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I /SM) and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number MZ484396) is a passaged variant of BVDV I NDAL strain (GenBank accession number AJ133738.1) [ 19 ] and propagated in MDBK … small usb music keyboardWebJul 19, 2024 · Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for the capacity to differentiate field CSFV isolates from CSF vaccine … small usb mouse for laptopWebMay 31, 2024 · A full-length cDNA clone of attenuated strain HuN4-F112, obtained by serial cell passage from HP-PRRSV HuN4-F5 (GenBank accession no. EF635006), was used as the backbone for insertion of the E2 gene from the CSFV Shimen strain (GenBank accession no. AF092448) [24]. The resultant recombinant virus was compared to the … hik connect indiaWebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF vaccines in China. The HLJZZ2014 strain is a subgenotype 2.1d CSFV isolated in 2014 in China (Luo et al., 2016). The CSFV Shimen and HLJZZ2014 strains were propagated … hik connect how to share deviceWeb猪瘟病毒野毒株E2蛋白特异性单抗免疫抑制制备法的建立.pdf hik connect idWebMar 9, 2010 · For the specificity assay, the RNA from the cells infected with CSFV strains (C-strain, Shimen, QZ-07, and XS-08), PCV2, PRRSV, PRV, or mock-infected cells was extracted for the established RT-nPCR. Three independent experiments were performed to test the repeatability. Sensitivity was determined through serial dilutions of in vitro … hik connect india server addressWebJul 1, 2015 · The Shimen strain infection resulted in a significant activation of IFN regulatory factor IRF7 and suppression of IRF3. These data provided clues for … small usb port called