Fitc偶联annexin-v凋亡检测试剂盒
Web实验笔记丨流式检测细胞凋亡之Annexin V 检测细胞凋亡. Annexin V 是检测细胞凋亡的灵敏指标之一。. 它是一种磷脂结合蛋白,可以与早期凋亡细胞的胞膜结合,而细胞质膜的改变是细胞发生凋亡时最早的改变之一。. 在细胞发生凋亡时,膜磷脂酰丝氨酸 (PS) 由质膜 ... WebDocuments. 产品描述. 本试剂盒用于检测细胞凋亡早期的发生,其中Annexin V为胞内蛋白膜联蛋白家族成员,以钙离子依赖的方式选择性与磷脂酰丝氨酸 (PS)结合。. PS正常分布在细胞膜内侧,即与细胞浆相邻的一侧。. 在细胞发生凋亡的早期,不同类型的细胞都会把PS ...
Fitc偶联annexin-v凋亡检测试剂盒
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WebAnnexin-V(膜联蛋白-V)是一种分子量为35-36KD的Ca2+依赖性磷脂结合蛋白,能与PS高亲和力结合。. 用荧光素(FITC、Alexa Fluor488等)标记的Annexin-V作为探针,利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生;碘化丙啶 (propidine iodide, PI)是一种核酸染料,它不能透过 ... Web推荐的实验流程. FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required ...
WebAnnexin V是一种分子量为35-36kD的Ca2 + 依赖性磷脂结合蛋白,能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸(PS)高亲和力特异性结合。在正常细胞中,磷脂酰丝氨酸(PS)只分布在细胞膜脂质双层的内侧,而在细胞凋亡早期,细胞膜中的磷脂酰丝氨酸(PS)由脂膜 ... WebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to …
WebGently mix and incubate at RT for 15 min in the dark. *For Annexin V-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1X Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 554060) into 100 µl of 1X Binding Buffer and add to the cell pellet. Gently mix the cells and incubate at RT for ... Web北京义翘神州(Sino Biological, Inc.)自主研发的高品质 Annexin V/7-AAD 凋亡检测试剂盒以 Annexin V 联合 7-AAD/PI 的双染法(提示 ...
WebANNEXIN V- FITC/PI 凋亡检测试剂盒用于细胞凋亡检测,Annexin V 是一种分子量为35.8 KD 的Ca2+依赖性磷脂结合蛋白,能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸(Phosphatidylserine, PS)高亲和力特异结合。
Web一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞,并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI),用于标记细胞膜完全破坏 … fish tank has bubbles at the topWebAnnexin V是检测细胞早期凋亡的常用方法之一。实验操作虽然不算难,想要获得漂亮的流式结果图却并不容易,任何一个细节的疏忽都可能导致实验结果不理想。 本篇文章,带大家了解实验过程中的注意事项,及不能忽视的实验细节。 01 实验原理 candy board for 60 birthdayWebAnnexin V是一种磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。. 因此Annexin V被作为检测细胞早期凋亡的灵敏指标之一。. 碘化丙啶(Propidium Iodide, PI)是一种核酸染料, 它不能透过完整的细胞膜 ... fish tank has lots of foam bubbles on topWebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X … fish tank has a line of bubbles at the topWebMay 27, 2024 · Annexin V-FITC细胞凋亡检测试剂盒(Annexin V-FITC Apoptosis Detection Kit)是用FITC标记的重组人Annexin V来检测细胞凋亡时出现在细胞膜表面的磷脂酰丝氨酸的一种细胞凋亡检测试剂盒。可以使用流式细胞仪、荧光显微镜或其它荧光检测设备进行检测。 candy boardmakerWebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. candy blondieWebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs ... fish tank hd